Askanas V, Engel W K, Ringel S P, Bender A N
Neurology. 1977 Nov;27(11):1019-22. doi: 10.1212/wnl.27.11.1019.
We describe the diffuse nonjunctional distribution of AChR molecules of aneurally cultured human and animal muscle and the influence of sera from myasthenia grivis patients and rabbits with experimental autoimmune myasthenia gravis on binding of alphaBT to diffuse nonjunctional AChR. One-hour incubation of myasthenia gravis seria resulted in blocking of the alphaBT-immunoperoxidase staining of their AChRs, while incubation in normal sera did not. Aneurally cultured muscle can aid studies of regenerating fibers in normal muscle compared with those of muscles in neuromuscular diseases, and also act as an environmentally controlled test object for demonstrating the effect of circulating pathogenic factors.
我们描述了无神经培养的人和动物肌肉中乙酰胆碱受体（AChR）分子的弥漫性非连接分布，以及重症肌无力患者和实验性自身免疫性重症肌无力兔的血清对α-银环蛇毒素（αBT）与弥漫性非连接AChR结合的影响。重症肌无力血清孵育1小时导致其AChR的αBT免疫过氧化物酶染色被阻断，而在正常血清中孵育则不会。与神经肌肉疾病中的肌肉相比，无神经培养的肌肉有助于研究正常肌肉中再生纤维，并且还可作为一个环境可控的测试对象，用于证明循环致病因子的作用。
