Aitken R J
J Embryol Exp Morphol. 1977 Oct;41:295-300.
When day-3 mouse embryos were cultured in a simple medium supplemented with uterine fluids of mice autopsied on day 4 of pregnancy, 48 h after administration of oestradiol, or during pro-oestrus, the percentage of blastocysts hatching from the zona pellucida was significantly greater than in unsupplemented medium. In the presence of uterine fluids recovered during delayed implantation this stimulation of blastocyst hatching was not observed. When the culture medium was supplemented with dialysed uterine flushings containing 20 or 30 microgram protein/ml, both 'day 4' and 'delay' uterine proteins were equally effective in enhancing hatching frequency (P less than 0.05). The results suggested that 'delay' uterine fluids may contain a dialysable inhibitor of blastocyst activity. The putative inhibitor was not effective in the presence of serum, since uterine fluids recovered both on day 4 of pregnancy and during delayed implantation significantly increased the size attained by blastocyst outgrowths in the presence of foetal calf serum (P less than 0.01). The percentage of blastocysts exhibitng giant cell transformation and outgrowth was also increased (P less than 0.02) by these uterine fluids when the concentration of FCS in the medium was minimal (0.25%).
将妊娠第3天的小鼠胚胎培养于添加了以下来源子宫液的简单培养基中时：在给予雌二醇48小时后、发情前期或在妊娠第4天解剖的小鼠的子宫液，从透明带孵化出的囊胚百分比显著高于未添加子宫液的培养基。在延迟着床期间回收的子宫液存在的情况下，未观察到对囊胚孵化的这种刺激作用。当培养基添加含有20或30微克蛋白质/毫升的透析子宫冲洗液时，“第4天”和“延迟”子宫蛋白在提高孵化频率方面同样有效（P<0.05）。结果表明，“延迟”子宫液可能含有一种可透析的囊胚活性抑制剂。假定的抑制剂在有血清存在时无效，因为在妊娠第4天和延迟着床期间回收的子宫液在有胎牛血清存在时显著增加了囊胚生长所达到的大小（P<0.01）。当培养基中胎牛血清浓度最低（0.25%）时，这些子宫液也增加了表现出巨细胞转化和生长的囊胚百分比（P<0.02）。
