一种新型循环反应系统的动力学及其在多核苷酸磷酸化酶或二磷酸腺苷加三磷酸腺苷测定中的应用。
The kinetics of a novel cyclic reaction system and its use in the assay of polynucleotide phosphorylase or adenosine diphosphate plus adenosine triphosphate.
作者信息
Wilson R J, Lilly M D
出版信息
Biochem J. 1968 Feb;106(4):887-95. doi: 10.1042/bj1060887.
Abstract
- A sensitive new method is described for the rapid optical assay of polynucleotide phosphorylase in crude bacterial extracts of low specific activity. 2. The assay is non-linear with time and a quick method for determining the approximate activity by using a nomograph is given. 3. The dependence of the assay on Mg(2+) concentration was investigated and the conditions that gave first-order kinetics were determined. 4. The theoretical behaviour of the system was examined mathematically and shown to tend to a simple dynamic steady state, under certain conditions. 5. The potential uses of such cyclic systems are discussed.
摘要
- 本文描述了一种灵敏的新方法，用于对低比活性的细菌粗提物中的多核苷酸磷酸化酶进行快速光学测定。2. 该测定随时间呈非线性，给出了一种使用列线图快速测定近似活性的方法。3. 研究了该测定对Mg(2+)浓度的依赖性，并确定了产生一级动力学的条件。4. 对该系统的理论行为进行了数学检验，结果表明在某些条件下趋向于一个简单的动态稳态。5. 讨论了这种循环系统的潜在用途。
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