烟酰胺核苷酸氧化型和还原型的测定
THE ESTIMATION OF THE OXIDIZED AND REDUCED FORMS OF THE NICOTINAMIDE NUCLEOTIDES.
作者信息
GREENBAUM A L, CLARK J B, MCLEAN P
出版信息
Biochem J. 1965 Apr;95(1):161-6. doi: 10.1042/bj0950161.
Abstract
- A method is described for the determination of the oxidized and reduced forms of the nicotinamide nucleotides by measuring the rate of the oxygen uptake with an oxygen electrode in a system in which the nucleotide acts as the rate-limiting carrier in a cyclic system. 2. The method permits the measurement of quantities as low as 0.02mug. of NAD(+) or NADH or 0.01mug. of NADP(+) or NADPH. 3. The method permits the measurement of the nucleotides in extracts that contain non-specific reducing substances, coloured compounds or fluorescent materials, e.g. green leaves. 4. The results obtained by the present method are compared with those reported in the literature.
摘要
- 本文描述了一种通过在一个循环系统中用氧电极测量氧气摄取速率来测定烟酰胺核苷酸氧化型和还原型的方法，在该系统中核苷酸作为限速载体。2. 该方法能够测量低至0.02微克的NAD(+)或NADH，或0.01微克的NADP(+)或NADPH。3. 该方法可用于测量含有非特异性还原物质、有色化合物或荧光物质（如绿叶）的提取物中的核苷酸。4. 将本方法得到的结果与文献报道的结果进行了比较。
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