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[Quantitative dansyl micromethod of amino acid analysis. Study of peptide maps of ribonuclease tryptic hydrolysate].

作者信息

Afanasenko G A, Nisanov V Ia, Shliapnikov S V, Kaminur L B, Severin E S

出版信息

Biokhimiia. 1977 Dec;42(12):2178-85.

PMID:563740
Abstract

Some variants of the quantitative dansyl method in combination with thin-layer chromatography on polyamide plates have been compared, and principles of its optimization have been demonstrated. Sensitivity of the method is about 10(-11) mole, the error does not exceed 10%. Data are given on the analysis by the dansyl method of peptides of the tryptic hydrolysate of bovine pancreatic ribonuclease after their separation by peptide mapping procedure.

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