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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Biochim Biophys Acta. 1978 Feb 27;528(2):239-46. doi: 10.1016/0005-2760(78)90198-4.

The relative contribution of propionate, and long-chain even-numbered fatty acids to the production of long-chain odd-numbered fatty acids in six pure cultures of rumen bacteria were studied, using single and double isotope procedures. 2. Both propionate and even-numbered fatty acids were converted to odd-numbered fatty acids, presumably by elongation of 2-C atoms and alpha-oxidation reactions, but even-numbered fatty acids were much more effective. 3. The occurrence of alpha-oxidation was further confirmed by relatively large quantities of 14CO2 produced when [1-14C]palmitate was used in the incubation medium. 4. The incorporation of propionate into odd-numbered fatty acids was markedly reduced in the presence of palmitate, or stearate. 5. The significance of the operation of alpha-oxidation is discussed in relation to the energy conservation in rumen bacteria.

采用单同位素和双同位素程序，研究了丙酸和长链偶数脂肪酸对六种瘤胃细菌纯培养物中长链奇数脂肪酸产生的相对贡献。2. 丙酸和偶数脂肪酸都可转化为奇数脂肪酸，推测是通过2 - 碳原子的延长和α - 氧化反应，但偶数脂肪酸的效果要好得多。3. 当在培养基中使用[1 - 14C]棕榈酸时，产生了相对大量的14CO2，这进一步证实了α - 氧化的发生。4. 在棕榈酸或硬脂酸存在的情况下，丙酸掺入奇数脂肪酸的量明显减少。5. 结合瘤胃细菌中的能量守恒，讨论了α - 氧化作用的意义。

Biochim Biophys Acta. 1978 Feb 27;528(2):239-46. doi: 10.1016/0005-2760(78)90198-4.

The relative contribution of propionate, and long-chain even-numbered fatty acids to the production of long-chain odd-numbered fatty acids in six pure cultures of rumen bacteria were studied, using single and double isotope procedures. 2. Both propionate and even-numbered fatty acids were converted to odd-numbered fatty acids, presumably by elongation of 2-C atoms and alpha-oxidation reactions, but even-numbered fatty acids were much more effective. 3. The occurrence of alpha-oxidation was further confirmed by relatively large quantities of 14CO2 produced when [1-14C]palmitate was used in the incubation medium. 4. The incorporation of propionate into odd-numbered fatty acids was markedly reduced in the presence of palmitate, or stearate. 5. The significance of the operation of alpha-oxidation is discussed in relation to the energy conservation in rumen bacteria.

采用单同位素和双同位素程序，研究了丙酸和长链偶数脂肪酸对六种瘤胃细菌纯培养物中长链奇数脂肪酸产生的相对贡献。2. 丙酸和偶数脂肪酸都可转化为奇数脂肪酸，推测是通过2 - 碳原子的延长和α - 氧化反应，但偶数脂肪酸的效果要好得多。3. 当在培养基中使用[1 - 14C]棕榈酸时，产生了相对大量的14CO2，这进一步证实了α - 氧化的发生。4. 在棕榈酸或硬脂酸存在的情况下，丙酸掺入奇数脂肪酸的量明显减少。5. 结合瘤胃细菌中的能量守恒，讨论了α - 氧化作用的意义。