Canosa C A, Rehm W S
Biophys J. 1968 Apr;8(4):415-30. doi: 10.1016/S0006-3495(68)86497-5.
In anesthetized dogs, the potentials in the mucous coat and gastic cells were measured with microelectrodes. In the secreting stomach, with isotonic saline in contact with the mucosal surface, the orientation of the initial change in potential difference (PD) was often the same as that of the liquid junction potential between gastric juice and saline (the microelectrode became negative to a reference electrode in the saline) but the magnitude of the change was never more than 11 mv. On the basis of this finding an explanation is offered for the observation that in the secreting stomach replacing isotonic saline with isotonic HCl as the bathing fluid on the mucosal surface, results in a change in the serosal to mucosal PD of only 19 mv, which is 40% less than the liquid junction potential between gastric juice and saline. In the surface epithelial cells of both resting and secreting stomach, multiple levels of potentials were found. For the secreting stomach, the resistance between the interstitial fluid of the pit region and the fluid on the mucosal surface was 55 ohm cm(2), determined as the change in PD per unit of applied current across stomach. The implications of these findings are discussed with reference to the separate site theory of HCl formation.
在麻醉犬身上，用微电极测量了胃黏膜层和胃细胞的电位。在分泌胃酸的胃中，当等渗盐水与黏膜表面接触时，电位差（PD）初始变化的方向通常与胃液和盐水之间的液体接界电位的方向相同（在盐水中，微电极相对于参比电极变为负极），但变化幅度从未超过11毫伏。基于这一发现，对以下观察结果给出了解释：在分泌胃酸的胃中，将黏膜表面的灌流液等渗盐水换成等渗盐酸时，浆膜与黏膜之间的PD变化仅为19毫伏，比胃液与盐水之间的液体接界电位低40%。在静息胃和分泌胃酸的胃的表面上皮细胞中，均发现了多个电位水平。对于分泌胃酸的胃，通过测量跨胃施加单位电流时PD的变化，确定胃小凹区域的细胞间液与黏膜表面液体之间的电阻为55欧姆·厘米²。结合盐酸形成的独立位点理论对这些发现的意义进行了讨论。
