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J Cell Biol. 1968 Apr;37(1):117-21. doi: 10.1083/jcb.37.1.117.
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10
The optical activity of bleached retinal receptors.漂白视网膜受体的旋光性。
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Protein conformation in cell membrane preparations as studied by optical rotatory dispersion and circular dichroism.通过旋光色散和圆二色性研究细胞膜制剂中的蛋白质构象。
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THE EFFECT OF THE FIXATIVE SOLUTION ON THE ULTRASTRUCTURE OF CELLS AND TISSUES. A COMPARATIVE ANALYSIS WITH PARTICULAR ATTENTION TO THE PROXIMAL CONVOLUTED TUBULE OF THE RAT KIDNEY.固定液对细胞和组织超微结构的影响。以大鼠肾脏近端曲管为重点的比较分析。
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STUDIES ON THE REACTION OF OSMIUM TETROXIDE WITH LIPIDS AND RELATED COMPOUNDS.四氧化锇与脂质及相关化合物反应的研究
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RELATIVE CONFORMATIONS OF SPERM WHALE METMYOGLOBIN AND APOMYOGLOBIN IN SOLUTION.溶液中抹香鲸高铁肌红蛋白和脱辅基肌红蛋白的相对构象
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电子显微镜所用固定剂对红细胞膜及溶液中蛋白质构象的改变。

Alteration of the conformation of proteins in red blood cell membranes and in solution by fixatives used in electron microscopy.

作者信息

Lenard J, Singer S J

出版信息

J Cell Biol. 1968 Apr;37(1):117-21. doi: 10.1083/jcb.37.1.117.

DOI:10.1083/jcb.37.1.117
PMID:5645836
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2107400/
Abstract

The effects of several commonly employed fixatives on the three-dimensional conformations of two soluble proteins and the protein of intact red blood cell membranes have been studied by means of circular dichroism measurements in the spectral region of the peptide absorption bands. The fixatives used produced significant and parallel conformational changes in all of the proteins, in the increasing order: glutaraldehyde; OsO(4); glutaraldehyde followed by OsO(4); and KMnO(4). The last two treatments obliterated most of the helical character of the proteins. The significance of these observations to the preparation of specimens for electron microscopy is discussed.

摘要

通过在肽吸收带光谱区域进行圆二色性测量，研究了几种常用固定剂对两种可溶性蛋白质以及完整红细胞膜蛋白三维构象的影响。所用固定剂使所有蛋白质产生了显著且平行的构象变化，变化程度依次递增：戊二醛；四氧化锇；先戊二醛后四氧化锇；以及高锰酸钾。最后两种处理方式消除了蛋白质的大部分螺旋特征。本文讨论了这些观察结果对电子显微镜标本制备的意义。