电子显微镜所用固定剂对红细胞膜及溶液中蛋白质构象的改变。
Alteration of the conformation of proteins in red blood cell membranes and in solution by fixatives used in electron microscopy.
作者信息
Lenard J, Singer S J
出版信息
J Cell Biol. 1968 Apr;37(1):117-21. doi: 10.1083/jcb.37.1.117.
Abstract
The effects of several commonly employed fixatives on the three-dimensional conformations of two soluble proteins and the protein of intact red blood cell membranes have been studied by means of circular dichroism measurements in the spectral region of the peptide absorption bands. The fixatives used produced significant and parallel conformational changes in all of the proteins, in the increasing order: glutaraldehyde; OsO(4); glutaraldehyde followed by OsO(4); and KMnO(4). The last two treatments obliterated most of the helical character of the proteins. The significance of these observations to the preparation of specimens for electron microscopy is discussed.
摘要
通过在肽吸收带光谱区域进行圆二色性测量，研究了几种常用固定剂对两种可溶性蛋白质以及完整红细胞膜蛋白三维构象的影响。所用固定剂使所有蛋白质产生了显著且平行的构象变化，变化程度依次递增：戊二醛；四氧化锇；先戊二醛后四氧化锇；以及高锰酸钾。最后两种处理方式消除了蛋白质的大部分螺旋特征。本文讨论了这些观察结果对电子显微镜标本制备的意义。
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Alteration of the conformation of proteins in red blood cell membranes and in solution by fixatives used in electron microscopy.电子显微镜所用固定剂对红细胞膜及溶液中蛋白质构象的改变。
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