Brinley F J
J Gen Physiol. 1968 Apr;51(4):445-77. doi: 10.1085/jgp.51.4.445.
Sodium and potassium influxes and outfluxes have been studied in single isolated muscle fibers from the giant barnacle both by microinjection and by external loading. The sodium influxes and outfluxes were 49 and 39 pmoles /cm(2)-sec (temperature = 15-16 degrees C) respectively. The potassium influxes and outfluxes were 28 and 60 pmoles/cm(2)-sec (temperature = 13-16 degrees C) respectively. Replacement of external sodium by lithium reduced sodium outflux by 67% but had no effect on potassium outflux. Removal of external potassum reduced the sodium outflux by 51% but had no effect on potassium outflux. External strophanthidin (10-30 microM) reduced sodium outflux by 80-90% and increased potassium outflux by 40% in normal fibers. The time constant for sodium exchange increased linearly with internal sodium concentration, as did the fraction of sodium outflux insensitive to a maximally inhibitory concentration of external strophanthidin in the range of 10 tO 80 mM internal sodium. The strophanthidin-sensitive component of sodium outflux could be related to the internal sodium concentration by the following empirical formula: See PDF for Equation
已通过微量注射和外部加载两种方法，对来自巨型藤壶的单个分离肌肉纤维中的钠和钾的流入与流出进行了研究。钠的流入和流出分别为49和39皮摩尔/平方厘米·秒（温度 = 15 - 16摄氏度）。钾的流入和流出分别为28和60皮摩尔/平方厘米·秒（温度 = 13 - 16摄氏度）。用锂替代外部钠使钠流出减少了67%，但对钾流出没有影响。去除外部钾使钠流出减少了51%，但对钾流出没有影响。在正常纤维中，外部毒毛花苷（10 - 30微摩尔）使钠流出减少80 - 90%，并使钾流出增加40%。钠交换的时间常数随内部钠浓度呈线性增加，在10至80毫摩尔内部钠范围内，对外部毒毛花苷最大抑制浓度不敏感的钠流出部分也是如此。钠流出的毒毛花苷敏感成分可通过以下经验公式与内部钠浓度相关：见PDF中的公式
