New synthetic medium for the production of Alternaria allergens.

Mold belonging to Genus Alternaria was isolated from the Tehran atmosphere. Isolates were cultivated on both synthetic and modified Sabouraud media. Extracts obtained from the two cultures and the un-inoculated media were separately dialyzed, sterilized and standardized. Skin testing of the hypersensitive individuals by the above extracts indicated that allergenic potency of the extract obtained from mold grown on synthetic medium was better than that grown on modified Sabouraud medium, with no false positive skin reaction. The usage of this synthetic medium for the production of allergenic mold extracts is suggested.