Grant G A, Han Y W, Anderson A W
Appl Environ Microbiol. 1978 Mar;35(3):549-53. doi: 10.1128/aem.35.3.549-553.1978.
Semisolid fermentation of ryegrass straw to increase its animal feed value was successfully performed on a pilot scale. The pilot plant, which could handle 100 kg of straw per batch, was designed so that all major operations could take place in one vessel. The straw was hydrolyzed at 121 degrees C for 30 min with 0.5 N H2SO4 (7:3 liquid:solid), treated with ammonia to raise the pH to 5.0, inoculated with Candida utilis, and fermented in a semisolid state (70% moisture). During fermentation the straw was held stationary with air blown up through it. Batch fermentation times were 12 to 29 h. Semisolid fermentation did not require agitation and supported abundant growth at 20 to 40 degrees C even at near zero oxygen tensions. Fermentation increased the protein content, crude fat content, and in vitro rumen digestibility of the straw.
黑麦草秸秆的半固态发酵在中试规模上成功进行，以提高其动物饲料价值。该中试工厂每批可处理100千克秸秆，其设计使得所有主要操作都能在一个容器中进行。秸秆在121摄氏度下用0.5N硫酸（液固比7:3）水解30分钟，用氨处理将pH值提高到5.0，接种产朊假丝酵母，并在半固态（70%水分）状态下发酵。发酵过程中秸秆保持静止，通过向其吹气。分批发酵时间为12至29小时。半固态发酵不需要搅拌，即使在接近零氧张力的情况下，在20至40摄氏度也能支持大量生长。发酵提高了秸秆的蛋白质含量、粗脂肪含量和体外瘤胃消化率。
