Han Y W, Anderson A W
Agricultural Research Service, U.S. Department of Agriculture, Oregon State University, Corvallis, Oregon 97331.
Appl Microbiol. 1975 Dec;30(6):930-4. doi: 10.1128/am.30.6.930-934.1975.
Candida utilis, Aureobasidium pullulans, and Trichoderma viride were grown on pretreated ryegrass straw. The pretreatment consisted of hydrolysis of straw with 0.5 N H(2)SO(4) (water-substrate, 3:1) at 121 C, 100 C, and room temperature and adjustment of the hydrolysate to pH 4.5 to 5.0 with 5 N NH(4)OH. The 121 C pretreatment yielded a material containing 30% sugar and 2.3% N. The fermentation was carried on semisolid substrate (moisture level, 75%) in rotating jars for 2 to 3 days at room temperature. The organisms grew rapidly during the period from 18 to 42 h of incubation. During this period the number of microbial cells increased by 20- to 200-fold, and the level of NH(3)-N decreased from 1.3 to 0.9%. The fermentation resulted in a fourfold increase in protein, fivefold increase in crude fat, and 40% increase in the digestibility of straw. The best result in terms of increasing protein and digestibility of straw was obtained when C. utilis was grown on straw preheated at 121 C.
产朊假丝酵母、出芽短梗霉和绿色木霉在预处理的黑麦草秸秆上生长。预处理包括用0.5N硫酸（水与底物比例为3:1）在121℃、100℃和室温下对秸秆进行水解，并用5N氢氧化铵将水解产物的pH值调至4.5至5.0。121℃预处理得到的物料含糖30%、含氮2.3%。发酵在旋转瓶中的半固体底物（水分含量75%）上于室温下进行2至3天。在培养18至42小时期间，这些微生物生长迅速。在此期间，微生物细胞数量增加了20至200倍，氨氮水平从1.3%降至0.9%。发酵使蛋白质增加了四倍，粗脂肪增加了五倍，秸秆的消化率提高了40%。当产朊假丝酵母在121℃预热的秸秆上生长时，在提高秸秆蛋白质和消化率方面获得了最佳结果。
