Kimura J, Obata T, Okada H
Endocrinol Jpn. 1978 Feb;25(1):7-12. doi: 10.1507/endocrj1954.25.7.
The percentage of labeled cells in the uterine luminal epithelium of cycling mice showed the different zonal distributions at each stage of estrous cycle after cumulative labeling with 3H-thymidine for 36 hr. It was estimated that the proliferating fraction in the epithelium at proestrus, estrus, metestrus, and diestrus was 100%, 100%, 40% and 5%, respectively. The percentage of labeled cells in the uterine luminal epithelium of cycling mice treated with progesterone remained below 10% level for at least 20 hr after injections of progesterone. Total labeling was attained in the uterine epithelium of castrated mice by the administration of estradiol-17beta. On the other hand, the cell proliferation in the uterine epithelium of castrated mice treated with estradiol and progesterone was markedly suppressed and the percentage of labeled cells remained approximately at 35%. The remaining cell population, however, still showed the mitotic potency when mice received estradiol. It is suggested from this study that the effect of progesterone is to suppress the epithelial cell proliferation and transfer cells into resting cell fraction which is still evoked to proliferate as the effect of estradiol and that a key factor controlling epithelial proliferation in mouse uterus during the estrous cycle is proliferating fraction rather than cell cycle time.
用³H-胸腺嘧啶核苷累积标记36小时后，处于动情周期的小鼠子宫腔上皮中标记细胞的百分比在动情周期的每个阶段呈现出不同的区域分布。据估计，动情前期、动情期、动情后期和动情间期上皮中的增殖分数分别为100%、100%、40%和5%。给处于动情周期的小鼠注射孕酮后，子宫腔上皮中标记细胞的百分比在注射后至少20小时内保持在10%以下。通过给予17β-雌二醇，去势小鼠的子宫上皮实现了完全标记。另一方面，用雌二醇和孕酮处理的去势小鼠子宫上皮中的细胞增殖明显受到抑制，标记细胞的百分比约为35%。然而，当小鼠接受雌二醇时，其余细胞群体仍显示出有丝分裂能力。这项研究表明，孕酮的作用是抑制上皮细胞增殖，并将细胞转化为静止细胞部分，而静止细胞部分在雌二醇的作用下仍会被诱导增殖，并且在动情周期中控制小鼠子宫上皮增殖的关键因素是增殖分数而非细胞周期时间。
