Allan D, Duffus W P, Higgins D A
Immunology. 1968 Apr;14(4):575-81.
Following oral infection of a 12-week-old chicken with an overnight broth culture of , serum was removed on the 9th day and tested for the presence of haemagglutinating antibody using erythrocytes modified with a Westphal lipopolysaccharide extract of . The serum was found to have a high titre and on `Sephadex' fractionation and ultracentrifugal preparation showed a marked IgM response. Spectrophotometric measurements were made of interactions between the O-antigen extract and the whole serum or its component IgM and IgG fractions. Hapten—antibody complexes were measurable with the whole serum and the IgM fraction, but no complexes were detected with the IgG fraction under the conditions of the experiment.
用的过夜肉汤培养物对12周龄的鸡进行口服感染后，在第9天采集血清，使用用的韦斯特法尔脂多糖提取物修饰的红细胞检测血凝抗体的存在。发现血清具有高滴度，并且在“葡聚糖凝胶”分级分离和超速离心制备中显示出明显的IgM反应。对O抗原提取物与全血清或其组成的IgM和IgG组分之间的相互作用进行了分光光度测量。在全血清和IgM组分中可检测到半抗原-抗体复合物，但在实验条件下，IgG组分中未检测到复合物。
