噬菌体f2和Q-β的RNA末端序列比较：化学及沉降平衡研究

Comparison of RNA terminal sequences of phages f2 and Q-beta: chemical and sedimentation equilibrium studies.

作者信息

Weith H L, Asteriadis G T, Gilham P T

出版信息

Science. 1968 Jun 28;160(3835):1459-60. doi: 10.1126/science.160.3835.1459.

DOI:10.1126/science.160.3835.1459

Abstract

The terminal fragment obtained by hydrolysis with ribonuclease T(1) of the ribonucleic acid from the bacteriophage Qbeta has been isolated and purified. The results of chemical and enzymatic hydrolysis of this fragment and of the intact RNA itself indicate that the fragment has the composition (10 Cp, 4 Up), and that the RNA has the terminal sequence -GP(9 Cp, 4 Up) CpA. These conclusions are supported by the results of an application of the sedimentation-equilibrium method in which the molecular weight of the Qbeta fragment was compared with that of the corresponding fragment from f2 phage RNA for which the terminal sequence, -GpUpUpApCpCpApCpCpCpA had previously been determined.

摘要

通过用核糖核酸酶T(1)水解噬菌体Qβ的核糖核酸而获得的末端片段已被分离和纯化。对该片段以及完整RNA本身进行化学和酶促水解的结果表明，该片段的组成为(10个胞嘧啶核苷酸，4个尿嘧啶核苷酸)，并且RNA具有末端序列-GP(9个胞嘧啶核苷酸，4个尿嘧啶核苷酸)CpA。沉降平衡法的应用结果支持了这些结论，在该方法中，将Qβ片段的分子量与f2噬菌体RNA的相应片段的分子量进行了比较，f2噬菌体RNA的末端序列-GpUpUpApCpCpApCpCpCpA先前已被确定。

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引用本文的文献

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The nucleotide sequence at the 5'-terminus of the Q RNA minus trand.Q RNA负链5'-末端的核苷酸序列。

Proc Natl Acad Sci U S A. 1970 Oct;67(2):921-8. doi: 10.1073/pnas.67.2.921.

2

Characterization of the subunit structure of the ribonucleic acid genome of influenza virus.流感病毒核糖核酸基因组亚基结构的表征

J Virol. 1971 Nov;8(5):701-7. doi: 10.1128/JVI.8.5.701-707.1971.

3

The in vitro addition of a polyadenylate sequence to the 3' end of phage Qbeta RNA and the biological activity of the product.在噬菌体Qβ RNA的3'末端体外添加聚腺苷酸序列及其产物的生物活性。

Proc Natl Acad Sci U S A. 1975 Feb;72(2):428-32. doi: 10.1073/pnas.72.2.428.

4

Site-directed mutagenesis: effect of an extracistronic mutation on the in vitro propagation of bacteriophage Qbeta RNA.定点诱变：顺反子外突变对噬菌体Qβ RNA体外增殖的影响

Proc Natl Acad Sci U S A. 1975 Jan;72(1):367-71. doi: 10.1073/pnas.72.1.367.

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