幼鼠皮肤中糖胺聚糖生物合成中间产物的组织含量及转换率
The tissue content and turnover rates of intermediates in the biosynthesis of glycosaminoglycans in young rat skin.
作者信息
Hardingham T E, Phelps C F
出版信息
Biochem J. 1968 Jun;108(1):9-16. doi: 10.1042/bj1080009.
Abstract
- The tissue contents of hexose monophosphate, N-acetylglucosamine 6-phosphate, UDP-glucose, UDP-galactose, UDP-N-acetylglucosamine, UDP-N-acetylgalactosamine and UDP-glucuronic acid were determined in the skin of young rats less than 1 day post partum. Tissue-space determinations were used to calculate their average cellular concentrations. 2. The incorporation of [U-(14)C]-glucose into the intermediates was recorded with time and their rates of turnover were calculated. The results demonstrated product-precursor relationships along the pathway of hexosamine synthesis and that of hexuronic acid synthesis. The rates of synthesis of UDP-N-acetylhexosamine and UDP-glucuronic acid were 1.5+/-0.3 and 0.24+/-0.03mmumoles/min./g. of tissue respectively. These results indicated the average turnover time of the total tissue glycosaminoglycans to be about 5 days.
摘要
- 测定了产后不到1天的幼鼠皮肤中磷酸己糖、6-磷酸-N-乙酰葡糖胺、尿苷二磷酸葡萄糖（UDP-葡萄糖）、尿苷二磷酸半乳糖（UDP-半乳糖）、尿苷二磷酸-N-乙酰葡糖胺、尿苷二磷酸-N-乙酰半乳糖胺和尿苷二磷酸葡糖醛酸的组织含量。采用组织间隙测定法计算它们的平均细胞浓度。2. 记录了[U-(14)C]-葡萄糖随时间掺入中间产物的情况，并计算了它们的周转速率。结果证明了己糖胺合成途径和糖醛酸合成途径中产物与前体的关系。尿苷二磷酸-N-乙酰己糖胺和尿苷二磷酸葡糖醛酸的合成速率分别为1.5±0.3和0.24±0.03微摩尔/分钟/克组织。这些结果表明整个组织糖胺聚糖的平均周转时间约为5天。
相似文献
1
The tissue content and turnover rates of intermediates in the biosynthesis of glycosaminoglycans in young rat skin.幼鼠皮肤中糖胺聚糖生物合成中间产物的组织含量及转换率
Biochem J. 1968 Jun;108(1):9-16. doi: 10.1042/bj1080009.
2
The biosynthesis in vitro of chondroitin sulphate in neonatal rat epiphysial cartilage.新生大鼠骨骺软骨中硫酸软骨素的体外生物合成
Biochem J. 1972 Jan;126(2):417-32. doi: 10.1042/bj1260417.
3
Uridine diphosphate glucuronic acid production and utilization in various tissues actively synthesizing glycosaminoglycans.在各种积极合成糖胺聚糖的组织中尿苷二磷酸葡糖醛酸的产生与利用。
Biochem J. 1972 Jun;128(2):215-27. doi: 10.1042/bj1280215.
4
The biosynthesis of galactofuranosyl residues in galactocarolose.半乳卡罗糖中呋喃半乳糖基残基的生物合成。
Biochem J. 1971 Mar;122(1):49-57. doi: 10.1042/bj1220049.
5
Biosynthesis of UDP-sugars from 32P-labelled precursors in various biological materials.利用32P标记前体在各种生物材料中进行UDP-糖的生物合成。
Comp Biochem Physiol. 1969 Aug 1;30(3):561-7. doi: 10.1016/0010-406x(69)92025-8.
6
Studies on the mechanism of galactosamine-1-phosphate and its inhibition of UDP-glucose pyrophosphorylase.半乳糖胺-1-磷酸的作用机制及其对尿苷二磷酸葡萄糖焦磷酸化酶抑制作用的研究。
Eur J Biochem. 1969 Sep;10(2):219-25. doi: 10.1111/j.1432-1033.1969.tb00677.x.
7
Biosynthesis of the glucuronic acid unit of hemicellulose B from UDP-glucuronic acid.由尿苷二磷酸葡萄糖醛酸合成半纤维素B的葡萄糖醛酸单元。
Biochim Biophys Acta. 1967 Nov 28;148(2):572-4. doi: 10.1016/0304-4165(67)90161-4.
8
Turnover of UDP-sugars in E.大肠杆菌中尿苷二磷酸糖的周转
Arch Biochem Biophys. 1969 Nov;134(2):612-22. doi: 10.1016/0003-9861(69)90325-7.
9
Role of acetylglucosamine in chitin synthesis in crayfish. II. Enzymes in the epidermis for incorporation of acetylglucosamine into UDP-acetylglucosamine.乙酰葡糖胺在小龙虾几丁质合成中的作用。II. 表皮中负责将乙酰葡糖胺掺入UDP-乙酰葡糖胺的酶。
Comp Biochem Physiol B. 1973 Aug 15;45(4):777-84. doi: 10.1016/0305-0491(73)90139-9.
10
Biosynthesis of glycosaminoglycans in bovine cornea. The effet of uridine diphosphate xylose.牛角膜中糖胺聚糖的生物合成。尿苷二磷酸木糖的作用。
Biochem J. 1970 Dec;120(4):719-23. doi: 10.1042/bj1200719.
引用本文的文献
1
UDP-Glycosyltransferase 3A Metabolism of Polycyclic Aromatic Hydrocarbons: Potential Importance in Aerodigestive Tract Tissues.尿苷二磷酸糖基转移酶 3A 对多环芳烃的代谢：在气消化道组织中的潜在重要性。
Drug Metab Dispos. 2020 Mar;48(3):160-168. doi: 10.1124/dmd.119.089284. Epub 2019 Dec 13.
2
Xenobiotica-metabolizing enzymes in the skin of rat, mouse, pig, guinea pig, man, and in human skin models.大鼠、小鼠、猪、豚鼠、人类和人皮肤模型中的皮肤外源物质代谢酶。
Arch Toxicol. 2018 Aug;92(8):2411-2456. doi: 10.1007/s00204-018-2232-x. Epub 2018 Jun 18.
3
Xenobiotic-metabolizing enzymes in the skin of rat, mouse, pig, guinea pig, man, and in human skin models.大鼠、小鼠、猪、豚鼠、人类皮肤以及人类皮肤模型中的异生物质代谢酶。
Arch Toxicol. 2014 Dec;88(12):2135-90. doi: 10.1007/s00204-014-1382-8. Epub 2014 Nov 5.
4
Studies on the control of hexosamine biosynthesis by glucosamine synthetase.关于氨基葡萄糖合成酶对己糖胺生物合成的调控研究。
Biochem J. 1971 Feb;121(4):711-20. doi: 10.1042/bj1210711.
5
The concentrations of sugar nucleotides in bovine corneal epithelium and endothelium.牛角膜上皮和内皮中糖核苷酸的浓度。
Biochem J. 1972 May;127(5):911-2. doi: 10.1042/bj1270911.
6
Distribution of biologically labelled radioactive hyaluronic acid injected into joints.注入关节的生物标记放射性透明质酸的分布情况。
Ann Rheum Dis. 1973 Mar;32(2):103-11. doi: 10.1136/ard.32.2.103.
7
Uridine diphosphate glucuronic acid production and utilization in various tissues actively synthesizing glycosaminoglycans.在各种积极合成糖胺聚糖的组织中尿苷二磷酸葡糖醛酸的产生与利用。
Biochem J. 1972 Jun;128(2):215-27. doi: 10.1042/bj1280215.
8
The excretion and degradation of chondroitin 4-sulphate administered to guinea pigs as free chondroitin sulphate and as proteoglycan.以游离硫酸软骨素和蛋白聚糖形式给予豚鼠的硫酸软骨素4-硫酸盐的排泄与降解
Biochem J. 1972 Nov;130(2):597-606. doi: 10.1042/bj1300597.
9
The biosynthesis in vitro of keratan sulphate in bovine cornea.牛角膜中硫酸角质素的体外生物合成
Biochem J. 1972 Jun;128(2):205-13. doi: 10.1042/bj1280205.
10
The biosynthesis in vitro of chondroitin sulphate in neonatal rat epiphysial cartilage.新生大鼠骨骺软骨中硫酸软骨素的体外生物合成
Biochem J. 1972 Jan;126(2):417-32. doi: 10.1042/bj1260417.
本文引用的文献
1
THE ENZYMATIC SYNTHESIS IN VITRO OF HYALURONIC ACID CHAINS.透明质酸链的体外酶促合成
Proc Natl Acad Sci U S A. 1955 May 15;41(5):253-60. doi: 10.1073/pnas.41.5.253.
2
A submicrodetermination of glucose.葡萄糖的亚微量测定
J Biol Chem. 1949 Nov;181(1):149-51.
3
A new spectrophotometric method for the detection and determination of keto sugars and trioses.一种用于检测和测定酮糖及丙糖的新分光光度法。
J Biol Chem. 1951 Oct;192(2):583-7.
4
Effect of epinephrine on rat diaphragm.肾上腺素对大鼠膈肌的作用。
J Biol Chem. 1950 Dec;187(2):769-76.
5
6
DETERMINATION OF GLUCOSAMINE AND GALACTOSAMINE USING BORATE BUFFERS FOR MODIFICATION OF THE ELSON-MORGAN AND MORGAN-ELSON REACTIONS.使用硼酸盐缓冲液修饰埃尔森-摩根反应和摩根-埃尔森反应来测定氨基葡萄糖和半乳糖胺。
Anal Biochem. 1964 Nov;9:253-62. doi: 10.1016/0003-2697(64)90183-6.
7
THE FEEDBACK CONTROL OF SUGAR NUCLEOTIDE BIOSYNTHESIS IN LIVER.肝脏中糖核苷酸生物合成的反馈控制
Proc Natl Acad Sci U S A. 1964 Aug;52(2):371-9. doi: 10.1073/pnas.52.2.371.
8
A modified uronic acid carbazole reaction.一种改良的糖醛酸咔唑反应。
Anal Biochem. 1962 Oct;4:330-4. doi: 10.1016/0003-2697(62)90095-7.
9
The precipitation of polyanions by long-chain aliphatic ammonium compounds. IV. Elution in salt solutions of mucopolysaccharide-quaternary ammonium complexes adsorbed on a support.长链脂肪族铵化合物对聚阴离子的沉淀作用。IV. 吸附于载体上的粘多糖 - 季铵络合物在盐溶液中的洗脱
Biochim Biophys Acta. 1961 Dec 9;54:213-26. doi: 10.1016/0006-3002(61)90360-2.
Zotero 插件