关于氨基葡萄糖合成酶对己糖胺生物合成的调控研究。
Studies on the control of hexosamine biosynthesis by glucosamine synthetase.
作者信息
Winterburn P J, Phelps C F
出版信息
Biochem J. 1971 Feb;121(4):711-20. doi: 10.1042/bj1210711.
Abstract
- The nature of the feedback inhibition of hexosamine biosynthesis on rat liver glucosamine synthetase (l-glutamine-d-fructose 6-phosphate aminotransferase, EC 2.6.1.16) by UDP-N-acetylglucosamine was investigated in detail. 2. Further modifiers of physiological importance are described. Glucose 6-phosphate and AMP potentiated the UDP-N-acetylglucosamine inhibition, and UTP behaved as an activator. These three compounds only exerted their action when the feedback inhibitor was bound to the enzyme. 3. ATP also inhibited the enzyme. 4. The actions of these various effectors are discussed in kinetic terms. 5. An interpretation of these findings with reference to the regulation of hexosamine biosynthesis is presented.
摘要
- 详细研究了UDP-N-乙酰葡糖胺对大鼠肝脏葡糖胺合成酶（L-谷氨酰胺-D-果糖6-磷酸氨基转移酶，EC 2.6.1.16）的己糖胺生物合成反馈抑制的性质。2. 描述了其他具有生理重要性的调节剂。6-磷酸葡萄糖和AMP增强了UDP-N-乙酰葡糖胺的抑制作用，而UTP起激活剂的作用。这三种化合物仅在反馈抑制剂与酶结合时才发挥作用。3. ATP也抑制该酶。4. 从动力学角度讨论了这些不同效应物的作用。5. 提出了参照己糖胺生物合成调节对这些发现的解释。
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