Johnson J H, Rosecrans J A
Res Commun Chem Pathol Pharmacol. 1978 Mar;19(3):435-44.
The effect of an ovulation-blocking methadone treatment (5mg/kg s.c. at 13:00, 14:00 and 15:00) on preovulatory LH release was studied in Wistar and Sprague-Dawley rats. Estrous cycles were followed by vaginal smears, and on the morning of proestrus a polyvinyl cannula was implanted in the right atrium via the external jugular vein so that blood samples could be collected without the use of anesthesia. Whole blood samples (50 microliter in duplicate) collected every 30 minutes from 13:00 to 18:00 that afternoon were assayed for LH by double antibody radioimmunoassay. Surges of LH release were seen significantly less often in the methadone-treated rats than in untreated or saline-injected controls, supporting the conclusion that methadone exerts its ovulation-blocking effect by preventing the CNS-controlled stimulation of pituitary LH release.
在Wistar和Sprague-Dawley大鼠中研究了排卵阻断美沙酮治疗（下午13:00、14:00和15:00皮下注射5mg/kg）对排卵前促黄体生成素（LH）释放的影响。通过阴道涂片监测动情周期，在动情前期的早晨，经颈外静脉将聚乙烯插管植入右心房，以便在不使用麻醉的情况下采集血样。当天下午13:00至18:00每30分钟采集一次全血样本（一式两份，每份50微升），采用双抗体放射免疫分析法测定LH。与未治疗或注射生理盐水的对照组相比，美沙酮治疗组大鼠LH释放高峰出现的频率明显较低，这支持了美沙酮通过阻止中枢神经系统控制的垂体LH释放刺激来发挥其排卵阻断作用的结论。
