Stoĭka R S
Biokhimiia. 1978 Mar;43(3):467-72.
Isoenzyme patterns of lactatedehydrogenase (LDH, EC 1.1.1.27) were determined in the loach (Misgurnus fossilis) oocytes, unfertilized eggs, developing embryos and larvae, and the sensitivity of the isoenzymes to the effect of AgNO3, p-chlormercurybenzoate and high temperature were investigated. The presence of 4 phenotypes of LDH patterns which differed in the isozymes number, in their relative activity, in electrophoretic mobility in the polyacrylamide gel columnes and in the frequency of occurence in different populations of the investigated species is demonstrated. Before the hatching stage there appeared a new isozyme which was completely inhibited by AgNO3 and had the same electrophoretic mobility in all LDH phenotypes. During the larvae development the content of this LDH isozyme increased. Some isozymes, especially those which had low anodic mobility and decreased in their relative quantity, disappeared during the development of embryos and larvae, p-Chlormercurybenzoate in the concentration 1 mM and heating at 70 degrees C during 20 minutes did not significantly affect LDH activities in the loach eggs, embryos and larvae.
测定了泥鳅（Misgurnus fossilis）卵母细胞、未受精卵、发育中的胚胎和幼体中乳酸脱氢酶（LDH，EC 1.1.1.27）的同工酶模式，并研究了这些同工酶对硝酸银、对氯汞苯甲酸和高温作用的敏感性。结果表明，在所研究的物种中，存在4种LDH模式的表型，它们在同工酶数量、相对活性、在聚丙烯酰胺凝胶柱中的电泳迁移率以及在不同群体中的出现频率方面存在差异。在孵化阶段之前，出现了一种新的同工酶，它完全被硝酸银抑制，并且在所有LDH表型中具有相同的电泳迁移率。在幼体发育过程中，这种LDH同工酶的含量增加。一些同工酶，特别是那些阳极迁移率低且相对数量减少的同工酶，在胚胎和幼体发育过程中消失了。浓度为1 mM的对氯汞苯甲酸和在70摄氏度下加热20分钟对泥鳅卵、胚胎和幼体中的LDH活性没有显著影响。
