四倍体泥鳅（Misgurnus fossilis L.）羧酸酯酶-2表型的定性变化。
Qualitative changes in carboxylesterase-2 phenotypes of tetraploid loaches (Misgurnus fossilis L.).
作者信息
Ivanenkov V V
出版信息
Biochem Genet. 1983 Jun;21(5-6):595-608. doi: 10.1007/BF00484449.
We have shown in our previous work that in the tetraploid loach Misgurnus fossilis carboxylesterase-2 (E-2) phenotypes of embryos and early larvae are controlled by the alleles of the Est-2 locus. In the present work it has been established that the somatic tissues of many adult fish homozygous for one of the Est-2 alleles, unlike the tissues of embryos and larvae, reveal an additional fraction of E-2 with electrophoretic mobility corresponding to that of the second allozyme. The activity of this additional fraction of E-2 varies with the tissue and depends on the age of fish and maintenance conditions. Phenotypes of the same individual homozygous for one of the Est-2 alleles can be manifested as homozygous or heterozygous according to the activity of the additional E-2 fraction. The results obtained are consistent with the assumption that in the tetraploid species Misgurnus fossilis there are two E-2 loci with common alleles of identical electrophoretic mobility.
我们在之前的研究中表明，在四倍体泥鳅中，胚胎和早期幼体的羧酸酯酶-2（E-2）表型受Est-2基因座等位基因的控制。在本研究中，已确定许多成年鱼类的体细胞组织，对于Est-2等位基因之一是纯合的，与胚胎和幼体的组织不同，显示出另一部分E-2，其电泳迁移率与第二种同工酶的迁移率相对应。这一额外部分的E-2活性因组织而异，并取决于鱼的年龄和饲养条件。对于Est-2等位基因之一纯合的同一个体的表型，根据额外E-2部分的活性，可以表现为纯合或杂合。所得结果与以下假设一致，即在四倍体物种泥鳅中存在两个具有相同电泳迁移率共同等位基因的E-2基因座。
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