Bartos F, Bartos D, Olsen G D, Anderson B, Daves G D
Res Commun Chem Pathol Pharmacol. 1978 Apr;20(1):157-64.
Diastereomeric methadols were prepared by reduction of the methadone enantiomers. Resulting methadols, after purification and characterization by mass spectrometry, were succinylated. The hemisuccinyl derivatives were conjugated with thyroglobulin via carbodiimide condensation. The conjugates of d- and of 1-methadol hemisuccinyl-thyroglobulin were employed as the antigens for immunization of white New Zealand rabbits in order to obtain specific anti d- and anti 1-methadone antisera.
通过美沙酮对映体的还原反应制备了非对映体美沙醇。所得美沙醇经纯化并通过质谱表征后，进行琥珀酰化反应。半琥珀酰衍生物通过碳二亚胺缩合与甲状腺球蛋白偶联。将d-美沙醇半琥珀酰-甲状腺球蛋白和1-美沙醇半琥珀酰-甲状腺球蛋白的偶联物用作抗原，免疫白色新西兰兔，以获得特异性抗d-美沙酮和抗1-美沙酮抗血清。
