Steinert P, Yuspa S H
Science. 1978 Jun 30;200(4349):1491-3. doi: 10.1126/science.566466.
More than 70 percent of the urea-extractable proteins from mouse stratum corneum or from differentiated cells of mouse epidermis grown in culture are two proteins of molecular weight 68,000 (keratin I) and 60,000 (kerae are two proteins of molecular weight 68,000 (keratin 1) and 60,000 (keratin 2), which are present in equimolar amounts on polyacrylamide gels. These proteins are the subunits of the keratin filaments, because when isolated from stratum corneum or cells grown in culture they form native-type epidermal keratin filaments in vitro. These observations provide biochemical evidence that epidermal cells grown in culture synthesize the major differentiation products of the epidermis.
从小鼠角质层或培养的小鼠表皮分化细胞中提取的、可被尿素提取的蛋白质，超过70%是两种分子量分别为68,000（角蛋白I）和60,000（角蛋白II）的蛋白质，它们在聚丙烯酰胺凝胶上以等摩尔量存在。这些蛋白质是角蛋白丝的亚基，因为当从角质层或培养的细胞中分离出来时，它们在体外形成天然类型的表皮角蛋白丝。这些观察结果提供了生化证据，表明培养的表皮细胞合成了表皮的主要分化产物。
