Chomczyński P, Dragon A, Zwierzchowski L
Acta Biochim Pol. 1978;25(1):23-8.
The content of non-histone protein in epithelial cell chromatin from rabbit mammary gland increased during late pregnancy and early lactation up to 30%, while the histone content remained unchanged. No qualitative changes, as judged by polyacrylamide-gel electrophoresis, were found during accumulation of non-histone proteins at the onset of lactation. The chromatin isolated at lactation, assayed either by polylysine or Toluidine Blue binding, contained slightly more available DNA-phosphate than the chromatin isolated at pregnancy.
兔乳腺上皮细胞染色质中的非组蛋白含量在妊娠后期和泌乳早期增加了30%，而组蛋白含量保持不变。通过聚丙烯酰胺凝胶电泳判断，在泌乳开始时非组蛋白积累过程中未发现定性变化。通过聚赖氨酸或甲苯胺蓝结合测定，泌乳期分离的染色质比妊娠期分离的染色质含有略多的可利用DNA磷酸盐。
