Orłowska-Majdak M, Werulik B, Traczyk W Z
Acta Physiol Pol. 1978 Jan-Feb;29(1):9-16.
Release of oxytocin and Substance P-like peptides from the rat posterior pituitary lobe incubated in situ after infusion of hypertonic solution into the third cerebral ventricle. Acta Physiol. Pol., 1978, 29 (1): 9-16. The experiments were carried out on male rats. Under general urethane and chloralose anaesthesia the ventral surface of the hypothalamus and the pituitary gland were exposed by a transpharyngeal approach and the anterior pituitary lobe was entirely removed. The posterior lobe with the remaining neural and partially vascular connections with the hypothalamus was incubated in situ. Oxytocin and Substance P-like peptides were assayed biologically in six 20-min samples of the fluid outflow from the posterior pituitary lobe incubated in situ. At the beginning of collection of the fourth sample a hypertonic solution was infused into the third ventricle through a micropipette. This infusion increased significantly the release of oxytocin and insignificantly that of Substance P-like pepides from the posterior pituitary lobe into the incubation fluid.
向第三脑室注入高渗溶液后，大鼠垂体后叶原位孵育时催产素和P物质样肽的释放。《波兰生理学报》，1978年，29(1):9 - 16。实验在雄性大鼠身上进行。在氨基甲酸乙酯和氯醛糖全身麻醉下，经咽入路暴露下丘脑腹面和垂体，完全切除垂体前叶。将与下丘脑保持神经和部分血管连接的垂体后叶进行原位孵育。对垂体后叶原位孵育流出液的六个20分钟样本进行催产素和P物质样肽的生物学检测。在收集第四个样本开始时，通过微量移液器向第三脑室注入高渗溶液。该注入显著增加了垂体后叶向孵育液中催产素的释放，而P物质样肽的释放增加不显著。
