Goraca A, Tranda T, Traczyk W Z
Acta Physiol Pol. 1984 Sep-Dec;35(5-6):460-8.
The experiments were carried out with 2 groups of male rats under urethane-chloralose anaesthesia. In the 1st group the anterior pituitary lobe was removed and the posterior one was incubated in situ in McIlwain's solution. The incubating fluid was collected with methanol, centrifuged, and the supernatant was lyophilized. The lyophilized fluid incubating the posterior pituitary lobe in situ was filtered on a Sephadex G-15 column and fractions with cardiodepressant activity were passed through a SP Sephadex C-25 column. The cardiodepressant activity was tested on an isolated auricle of the right atrium of a two-day-old rat. The cardiodepressant activity of the fractions was evaluated. The fluid incubating the posterior pituitary lobe was found to contain a factor reducing the contraction rate of the isolated auricle. In the control group the sphenoid bone was exposed and bathed in McIlwain's solution, in the same approach as the posterior pituitary lobe. The fluid bathing the sphenoid bone in situ was filtered on a Sephadex G-15 column. No cardiodepressant activity was found in the fractions.
实验在乌拉坦-氯醛糖麻醉下的两组雄性大鼠身上进行。在第一组中，切除垂体前叶，将垂体后叶原位置于麦基尔韦恩溶液中孵育。用甲醇收集孵育液，离心，上清液冻干。将原位孵育垂体后叶的冻干液在葡聚糖凝胶G-15柱上过滤，具有心脏抑制活性的组分通过SP葡聚糖凝胶C-25柱。在新生两天大鼠的离体右心房耳上测试心脏抑制活性。评估各组分的心脏抑制活性。发现孵育垂体后叶的液体含有一种降低离体心房耳收缩率的因子。在对照组中，以与垂体后叶相同的方法暴露蝶骨并置于麦基尔韦恩溶液中。原位冲洗蝶骨的液体在葡聚糖凝胶G-15柱上过滤。各组分中未发现心脏抑制活性。
