由从大鼠胃黏膜分离的细胞悬液合成的特征性蛋白质。

Characteristic proteins systhesized by suspensions of cells isolated from rat gastric mucosa.

作者信息

Trotman C N, Sanderson C, Spink C

出版信息

Cell Biol Int Rep. 1978 Mar;2(2):177-84. doi: 10.1016/0309-1651(78)90039-5.

Abstract

The biochemical integrity of rat gastric mucosal cells after isolation with pronase was demonstrated by their synthesis of a wide range of proteins. Approximately 50 bands of pre-existing proteins were detectable by staining on SDS-polyacrylamide electrophoresis gels. Most bands appeared readiolabelled after incubating cells with 14C-leucine or 14C-proline and were detected by autoradiography and fluorography of the gels. Recent synthesis, indicated by the radioactivity of each band, was compared with historical synthesis indicated by the intensity of staining. Densitometric comparisons showed the relative specific radioactivity of certain bands, e.g., one of nominal molecular weight 25000, to be consistently higher than the integrated mean value.

摘要

用链霉蛋白酶分离后，大鼠胃黏膜细胞的生化完整性通过其对多种蛋白质的合成得以证明。在十二烷基硫酸钠-聚丙烯酰胺电泳凝胶上染色可检测到约50条预先存在的蛋白质条带。在用14C-亮氨酸或14C-脯氨酸孵育细胞后，大多数条带出现放射性标记，并通过凝胶的放射自显影和荧光自显影进行检测。将各条带的放射性所表明的近期合成与染色强度所表明的既往合成进行比较。光密度测定比较显示某些条带（例如，一种标称分子量为25000的条带）的相对比放射性始终高于积分平均值。