Goodridge A G
Biochem J. 1968 Jul;108(4):663-6. doi: 10.1042/bj1080663.
The activities of several enzymes possibly implicated in lipogenesis were measured in the soluble fraction of homogenates of liver and adipose tissue of embryonic and growing chicks. The activities of adipose-tissue enzymes showed little or no change. The activities of hepatic hexose monophosphate-shunt dehydrogenases, malate dehydrogenase, 3-phosphoglyceraldehyde dehydrogenase and NAD-linked alpha-glycerophosphate dehydrogenase also showed little or no change. Isocitrate dehydrogenase activity in liver rose to a peak on the day of hatching and fell to half the peak value during the next 12 days, where it remained to 26 days after hatching. The activities of ;malic' enzyme and citrate-cleavage enzyme showed very low stable values in embryonic liver and remarkable rises during the early part of the post-hatching period. An 85-fold increase in the activity of ;malic' enzyme activity was completed in 7 days and a 15-fold increase in that of citrate-cleavage enzyme in 5 days. The activities then attained were maintained up to 26 days after hatching. 2. The increases in the activities of hepatic citrate-cleavage enzyme and ;malic' enzyme occurred simultaneously with a marked increase in lipogenesis, suggesting a relationship of these enzymes to lipogenesis in chick liver. By contrast, activity of the hexose monophosphate-shunt dehydrogenases does not appear to be thus associated.
在胚胎期和生长发育期雏鸡的肝脏及脂肪组织匀浆的可溶性部分，测定了几种可能与脂肪生成有关的酶的活性。脂肪组织中酶的活性几乎没有变化或没有变化。肝脏中磷酸己糖支路脱氢酶、苹果酸脱氢酶、3-磷酸甘油醛脱氢酶和NAD连接的α-甘油磷酸脱氢酶的活性也几乎没有变化或没有变化。肝脏中的异柠檬酸脱氢酶活性在孵化当天升至峰值，并在接下来的12天内降至峰值的一半，在孵化后26天一直保持这一水平。“苹果酸”酶和柠檬酸裂解酶的活性在胚胎肝脏中显示出非常低的稳定值，在孵化后早期显著升高。“苹果酸”酶活性在7天内增加了85倍，柠檬酸裂解酶活性在5天内增加了15倍。然后达到的活性在孵化后26天一直保持。2. 肝脏柠檬酸裂解酶和“苹果酸”酶活性的增加与脂肪生成的显著增加同时发生，表明这些酶与雏鸡肝脏中的脂肪生成有关。相比之下，磷酸己糖支路脱氢酶的活性似乎没有这种关联。
