兔虹膜中溶酶体透明质酸酶的存在。
The presence of lysosomal hyaluronidase in the rabbit iris.
作者信息
Hayasaka S, Sears M L
出版信息
Invest Ophthalmol Vis Sci. 1978 Jul;17(7):639-44.
PMID:566732
Abstract
Lysosomal hyaluronidase in the rabbit iris was studied by means of a sensitive assay method based on carbocyanine dye binding. The enzyme activity in the lysosomal extract was proportional to both the enzyme concentration and the incubation time. When the enzyme was heated, the enzyme activity was lost completely. When the lysosomal extract was used as an enzyme source, the enzyme activity was protected by pepstatin. The enzyme had a pH optimum of 3.8 and no activity above pH 5.0.
摘要
采用基于羰花青染料结合的灵敏检测方法，对兔虹膜中的溶酶体透明质酸酶进行了研究。溶酶体提取物中的酶活性与酶浓度和孵育时间均成正比。当酶受热时，酶活性完全丧失。当使用溶酶体提取物作为酶源时，胃蛋白酶抑制剂可保护酶活性。该酶的最适pH值为3.8，在pH 5.0以上无活性。
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