Calvert H E, Lalonde M, Bhuvaneswari T V, Bauer W D
Can J Microbiol. 1978 Jul;24(7):785-93. doi: 10.1139/m78-132.
The binding of purified, ferritin-labeled soybean seed lectin to the cell surfaces of Rhizobium japonicum 31 lb 138 has been examined by whole mount, thin section, and freeze-etch electron microscopy. The ferritin-labeled lectin binds in a biochemically specific manner to the capsular material of this bacterium. The lectin does not bind to the outer membranes of the cells or to flagella. Labeled lectin binds to sites throughout the capsular structure, although the density of labeling is somewhat greater on the outer surface of the capsule. Some cells appear to be partially encapsulated. Preservation of the capsular material proved difficult, and methods for retaining most of the capsular material were developed.
已通过整装、超薄切片和冷冻蚀刻电子显微镜检查了纯化的、铁蛋白标记的大豆种子凝集素与日本根瘤菌31lb138细胞表面的结合情况。铁蛋白标记的凝集素以生化特异性方式与该细菌的荚膜物质结合。凝集素不与细胞的外膜或鞭毛结合。标记的凝集素结合在整个荚膜结构的位点上，尽管在荚膜外表面的标记密度略高一些。一些细胞似乎部分被荚膜包裹。荚膜物质的保存证明很困难，因此开发了保留大部分荚膜物质的方法。
