Yeoman L C, Taylor C W, Woolf L M, Busch H
Cancer. 1978 Aug;42(2):474-82. doi: 10.1002/1097-0142(197808)42:2<474::aid-cncr2820420215>3.0.co;2-f.
Two-dimensional polyacrylamide gel electrophoretic analysis was carried out on HeLa, KB, ALL and GW-39 cell "Chromatin Fraction II" proteins. Of the many proteins found, most were visualized in earlier studies on rodent tumors and normal tissues. Of these the greater density of protein CP and the presence of protein CG' differentiates tumors from the nontumor tissues. In samples of normal and mitogen stimulated human lymphocytes, protein CG' was absent and protein CP was present in small amounts. Two-dimensional patterns of 0.4N H2SO4 soluble nuclear proteins showed elevated amounts of proteins C16-18 in the GS-39 cell patterns. Proteins Hu1, G1, G2, G3 and G5 were detected only in human cell nuclei. The increased density of staining for protein CP and the presence of CG' are potential biological markers for neoplastic cells.
对HeLa、KB、ALL和GW - 39细胞的“染色质组分II”蛋白质进行了二维聚丙烯酰胺凝胶电泳分析。在所发现的众多蛋白质中，大多数在早期对啮齿动物肿瘤和正常组织的研究中就已可见。其中，蛋白质CP的密度更高以及蛋白质CG'的存在使肿瘤与非肿瘤组织得以区分。在正常和有丝分裂原刺激的人淋巴细胞样本中，不存在蛋白质CG'，且蛋白质CP含量很少。0.4N硫酸可溶核蛋白的二维图谱显示，GS - 39细胞图谱中蛋白质C16 - 18的含量升高。蛋白质Hu1、G1、G2、G3和G5仅在人细胞核中被检测到。蛋白质CP染色密度的增加以及CG'的存在是肿瘤细胞的潜在生物学标志物。
