Goodridge A G
Mol Cell Endocrinol. 1978 Jun;11(1):19-29. doi: 10.1016/0303-7207(78)90029-1.
Synthesis of malic enzyme was rapidly and markedly stimulated by the addition of triiodothyronine to chick embryo liver cells in culture. Alpha-Amanitin, an inhibitor of DNA-dependent RNA polymerase II, blocked induction. The kinetics of induction and de-induction of malic enzyme synthesis suggested that the most stable event in triiodothyronine induction had a half-life of 18 to 20 h. However, malic enzyme synthesis decayed with a half-life of 2,4 h when transcription was inhibited with alpha-amanitin. Thus a long-lived event in thyroid hormone stimulation of malic enzyme synthesis occurred prior to transcription of a specific messenger RNA (mRNA), presumably malic enzyme mRNA. Malic enzyme synthesis decayed with a half-life of about 2 h when glucagon was added to pre-induced liver cells. The similarity of decay rates after inhibition of transcription with alpha-amanitin or inhibition of malic enzyme synthesis by glucagon suggests that glucagon may inhibit the transcription or processing of a specific mRNA required for malic enzyme synthesis.
在培养的鸡胚肝细胞中添加三碘甲状腺原氨酸后，苹果酸酶的合成受到快速且显著的刺激。α-鹅膏蕈碱是一种依赖DNA的RNA聚合酶II的抑制剂，它能阻断这种诱导作用。苹果酸酶合成的诱导和去诱导动力学表明，三碘甲状腺原氨酸诱导过程中最稳定的事件半衰期为18至20小时。然而，当用α-鹅膏蕈碱抑制转录时，苹果酸酶的合成以2.4小时的半衰期衰减。因此，在甲状腺激素刺激苹果酸酶合成过程中，一个长寿命事件发生在特定信使RNA（mRNA）转录之前，推测该mRNA为苹果酸酶mRNA。当向预先诱导的肝细胞中添加胰高血糖素时，苹果酸酶的合成以约2小时的半衰期衰减。用α-鹅膏蕈碱抑制转录或用胰高血糖素抑制苹果酸酶合成后衰减速率的相似性表明，胰高血糖素可能抑制苹果酸酶合成所需特定mRNA的转录或加工。
