原代组织培养中的新生大鼠肝细胞不受生长的密度依赖性调节。
Neonatal rat hepatocytes in primary tissue culture free from density-dependent regulation of growth.
作者信息
Armato U, Andreis P G, Draghi E, Mengato L
出版信息
In Vitro. 1978 Jun;14(6):479-84. doi: 10.1007/BF02616087.
PMID:567183
Abstract
Studies employing [3H]thymidine and radioautography as well as colchicine and Feulgen staining of DNA showed that up to 19-fold increases in the degree of cell crowding in vitro, i.e. from 1.45 to 27.55 X 10(4) cells per specimen, did not change the rates of entry into DNA synthesis and mitosis of cultivated primary neonatal rat hepatocytes.
摘要
采用[3H]胸腺嘧啶核苷和放射自显影技术，以及秋水仙碱和DNA福尔根染色法的研究表明，体外细胞拥挤程度增加至19倍，即每个样本从1.45×10(4)个细胞增加到27.55×10(4)个细胞，并未改变原代新生大鼠培养肝细胞进入DNA合成期和有丝分裂期的速率。
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