铅的细胞与分子毒理学。I. 铅对培养细胞增殖的影响。

Cellular and molecular toxicology of lead. I. Effect of lead on cultured cell proliferation.

作者信息

Kusell M, O'Cheskey S, Gerschenson L E

出版信息

J Toxicol Environ Health. 1978 Jul;4(4):503-13. doi: 10.1080/15287397809529674.

Abstract

Growth studies were done on a cultured rat liver cell line (RLC-GAI) grown in a chemically defined medium in the presence of lead nitrate. Lead reversibly inhibited the growth of these cells even after 6 d of exposure to the heavy metal. To compare lead sensitivity in various cell lines, GI50 and LD50 values were determined in the RLC-GAI cells as well as two glioma cell lines (B82 and C(6)) and a neuroblastoma cell line (N18). The LD50 values paralleled but were consistently lower than the GI50 values. Since lead is known to affect heme synthesis, hemin was added to test the possibilty of preventing the growth-inhibitory effect of the lead. The growth capacity of lead-treated cells did not change with the addition of hemin. It is thought that differentiated cultured cell lines such as these could be useful in examining the molecular mechanism of lead toxicity.

摘要

在添加硝酸铅的化学限定培养基中培养大鼠肝癌细胞系（RLC-GAI），并进行生长研究。即使在暴露于重金属6天后，铅仍能可逆地抑制这些细胞的生长。为了比较不同细胞系对铅的敏感性，在RLC-GAI细胞以及两种胶质瘤细胞系（B82和C(6)）和一种神经母细胞瘤细胞系（N18）中测定了GI50和LD50值。LD50值与GI50值平行，但始终低于GI50值。由于已知铅会影响血红素合成，因此添加血红素以测试预防铅生长抑制作用的可能性。添加血红素后，铅处理细胞的生长能力没有变化。据认为，这样的分化培养细胞系可用于研究铅毒性的分子机制。