Green A E, Pegrum G D
Br Med J. 1968 Sep 7;3(5618):591-2. doi: 10.1136/bmj.3.5618.591.
A rapid technique suitable for routine pathology laboratories has been used to estimate methylmalonic acid excretion in a 24-hour urine collection following a 10g. valine load. Levels above 40 mg./24 hours were found only in patients with vitamin B(12) deficiency. Patients with pernicious anaemia treated more than 24 hours before urine collection and patients with other types of anaemia had methylmalonic acid levels below 25 mg./24 hours.This method of demonstrating vitamin B(12) deficiency can be applied rapidly in debilitated patients so that specific treatment can be instituted within 36 hours of admission.
一种适用于常规病理实验室的快速技术已被用于评估在给予10克缬氨酸负荷后24小时尿液收集物中的甲基丙二酸排泄量。仅在维生素B12缺乏的患者中发现水平高于40毫克/24小时。在尿液收集前24小时以上接受治疗的恶性贫血患者和其他类型贫血患者的甲基丙二酸水平低于25毫克/24小时。这种证明维生素B12缺乏的方法可以在体弱患者中快速应用，以便在入院后36小时内开始进行特异性治疗。
