Hew C L
Can J Biochem. 1978 Aug;56(8):791-3. doi: 10.1139/o78-120.
Poly(A)-containing ribonucleic acid from cod islet greatly stimulated the incorporation of radioactive amino acids into proteins when assayed in a wheat germ translation system. The translation products were examined by specific immunoprecipitation with guinea pig anti cod insulin antibodies and by extraction with acid--ethanol. These measurements revealed at least a fivefold increase in incorporation of labelled amino acid over the nonprogrammed system. Sodium dodecyl sulfate disc gel electrophoresis and gel filtration chromatography showed a product of molecular weight 12 500, a size considerably larger than cod proinsulin (9000). It is concluded that cod proinsulin is synthesized via a larger precursor, preproinsulin.
在小麦胚芽翻译系统中进行检测时，鳕鱼胰岛中含聚腺苷酸的核糖核酸极大地刺激了放射性氨基酸掺入蛋白质的过程。通过用豚鼠抗鳕鱼胰岛素抗体进行特异性免疫沉淀以及用酸 - 乙醇提取来检测翻译产物。这些测量结果显示，与非编程系统相比，标记氨基酸的掺入量至少增加了五倍。十二烷基硫酸钠圆盘凝胶电泳和凝胶过滤色谱显示出一种分子量为12500的产物，其大小比鳕鱼胰岛素原（9000）大得多。得出的结论是，鳕鱼胰岛素原是通过一种更大的前体——前胰岛素原合成的。
