Hale T W, Wenzel D G
Histochem J. 1978 Sep;10(5):505-15. doi: 10.1007/BF01003133.
A method is described for measuring the latency of lysosomal acid phosphatase in cultured rat heart endotheloid cells. 210Pb was added to a medium used to demonstrate acid phosphatase activity by the Gomori lead method, and the amount of lead deposited was measured with a liquid scintillation counter. Deposition rates were measured after enzyme activation pretreatments with acetate buffer (pH 5.0) at various osmolalities, and after formaldehyde fixation. Formaldehyde, alloxan, or fluoride in the Gomori medium were evaluated for their differential effects on lysosomal and non-lysosomal acid phosphatase. The method was found to provide a sensitive, rapid and quantitative evaluation of acid phosphatase latency and should be useful for studying the integrity of lysosomes within cells.
描述了一种测量培养的大鼠心脏内皮样细胞中溶酶体酸性磷酸酶潜伏期的方法。将210Pb添加到用于通过Gomori铅法证明酸性磷酸酶活性的培养基中，并用液体闪烁计数器测量沉积的铅量。在不同渗透压下用乙酸盐缓冲液（pH 5.0）进行酶激活预处理后以及甲醛固定后测量沉积速率。评估了Gomori培养基中的甲醛、四氧嘧啶或氟化物对溶酶体和非溶酶体酸性磷酸酶的不同影响。发现该方法可为酸性磷酸酶潜伏期提供灵敏、快速和定量的评估，并且对于研究细胞内溶酶体的完整性应该是有用的。
