Hänssler G, Maxwell D P, Maxwell M D
J Bacteriol. 1975 Nov;124(2):997-1006. doi: 10.1128/jb.124.2.997-1006.1975.
A lysosomal system was demonstrated in hyphal tip cells of Sclerotium rolfsii by light and electron microscopy observations of the sites of acid phosphatase activity visualized by a modified Gomori lead nitrate method. The cytochemical reaction product was found to be present in numerous vacuoles, each aout 0.5 mum in diameter, which were seen as chains of spheres when viewed with the light microscope. They usually did not occur in the first 30 to 40 mum of the hyphal tip cell, but were concentrated in a zone extending from 30 to 200 mum from the hyphal apex. As shown by the electron microscope, the vacuoles were sometimes interconnected by narrow channels. Acid phosphatase reaction product was also occasionally localized in vacuoles of the older hyphal cells, but never in apical vesicles, lipid bodies, or microbodies. It is proposed that this vacuolar system may orginate from the endoplasmic reticulum.
通过改良的Gomori硝酸铅法对酸性磷酸酶活性位点进行光镜和电镜观察，在齐整小核菌的菌丝尖端细胞中证实了一种溶酶体系统。细胞化学反应产物存在于众多液泡中，每个液泡直径约0.5μm，在光镜下观察时呈球形链状。它们通常不出现在菌丝尖端细胞的前30至40μm，但集中在从菌丝顶端延伸30至200μm的区域。如电子显微镜所示，液泡有时通过狭窄通道相互连接。酸性磷酸酶反应产物偶尔也定位在较老菌丝细胞的液泡中，但从未出现在顶端小泡、脂质体或微体中。有人提出，这种液泡系统可能起源于内质网。
