Javid J, Yingling W
J Clin Invest. 1968 Oct;47(10):2290-6. doi: 10.1172/JCI105914.
The various antigenic determinants of serum haptoglobins (Hp) may be studied semiquantitatively in a radioimmunoassay system. Using three antisera of overlapping specificities, we constructed an antigenic model of the various Hp phenotypes comprising four distinct determinants. This adds a new determinant, situated near the hemoglobin binding site, to the three determinants reported in the literature. The assay system is sensitive to 0.2 mug or less of serum Hp. Furthermore, it can detect determinants that are not recognized by double diffusion studies.
血清触珠蛋白(Hp)的各种抗原决定簇可在放射免疫分析系统中进行半定量研究。我们使用三种具有重叠特异性的抗血清,构建了一个包含四个不同决定簇的各种Hp表型的抗原模型。这在文献报道的三个决定簇之外,又增加了一个位于血红蛋白结合位点附近的新决定簇。该检测系统对0.2微克或更少的血清Hp敏感。此外,它还能检测到双向扩散研究无法识别的决定簇。