Lindl T, Heinl-Sawaya M C, Cramer H
Res Commun Chem Pathol Pharmacol. 1976 Jan;13(1):65-74.
The effect of compound 48/80 in various rat brain regions - by means of incubating brain sclices with compound 48/80 - on cyclic AMP accumulation and release of the nucleotide into the incubation medium was studied. In all regions tested, accumulation of cyclic AMP occurred within at least 10 min. In the cortex, brain stem and cerebellar regions these increases of cyclic AMP could be blocked by propranolol, a beta-adrenergic antagonist. Diphenhydramin, an antihistaminic agent, was without effect in these regions, but could partially block the compound 48/80-induced rise of cyclic AMP in the hypothalamus. Propranolol had in this region only a moderate blocking effect. Release of cyclic AMP, induced by compound 48/80, occurred in all regions tested and could not be effectively blocked by propranolol or diphenhydramin.
通过用化合物48/80孵育脑切片，研究了化合物48/80在大鼠不同脑区对环磷酸腺苷（cAMP）积累以及核苷酸释放到孵育培养基中的影响。在所有测试区域中，至少在10分钟内出现了cAMP的积累。在皮质、脑干和小脑区域，cAMP的这些增加可被β-肾上腺素能拮抗剂普萘洛尔阻断。抗组胺药苯海拉明在这些区域没有作用，但可部分阻断化合物48/80诱导的下丘脑cAMP升高。普萘洛尔在该区域只有适度的阻断作用。化合物48/80诱导的cAMP释放发生在所有测试区域，并且不能被普萘洛尔或苯海拉明有效阻断。
