Johansson K, Persson H, Lewis A M, Pettersson U, Tibbetts C, Philipson L
J Virol. 1978 Sep;27(3):628-39. doi: 10.1128/JVI.27.3.628-639.1978.
Complementary strand-specific adenovirus DNA of full length or from endonuclease BamHI fragments was used as a probe to estimate the fractional representation and abundance of viral sequences in five hamster cell lines (Ad2HE1-5) transformed with UV-inactivated adenovirus type 2. The fraction of the viral genome present in the five transformed cell lines varied from 44% in the Ad2HE5 cell line to 84% in the Ad2HE3 cell line. The number of viral DNA copies per diploid cell equivalent ranged from 1.8 in the Ad2HE1 line to 7.1 in the Ad2HE4 line. In vivo labeling with [35S]methionine followed by immunoprecipitation with an antiserum against adenovirus type 2 early proteins revealed virus-specific polypeptides with molecular weights of 42,000 to 58,000 in extracts from all five hamster cell lines. Several other early viral polypeptides were detected in some of the adenovirus type 2-transformed hamster cell lines.
使用全长或来自核酸内切酶BamHI片段的互补链特异性腺病毒DNA作为探针，来估计在五个用紫外线灭活的2型腺病毒转化的仓鼠细胞系（Ad2HE1 - 5）中病毒序列的分数表示和丰度。五个转化细胞系中存在的病毒基因组分数从Ad2HE5细胞系中的44%到Ad2HE3细胞系中的84%不等。每个二倍体细胞当量的病毒DNA拷贝数范围从Ad2HE1系中的1.8到Ad2HE4系中的7.1。用[35S]甲硫氨酸进行体内标记，然后用抗2型腺病毒早期蛋白的抗血清进行免疫沉淀，结果显示在所有五个仓鼠细胞系的提取物中都存在分子量为42,000至58,000的病毒特异性多肽。在一些2型腺病毒转化的仓鼠细胞系中还检测到了其他几种早期病毒多肽。
