Thomas C B, Kohn K W, Bonner W M
Biochemistry. 1978 Sep 19;17(19):3954-8. doi: 10.1021/bi00612a012.
Proteins cross-linked to DNA after nitrogen mustard (HN2) treatment of cells or isolated nuclei were purified in CsCl gradients. The protein-DNA cross-links could be cleaved by incubation in dilute acid and could be stabilized by alkali pretreatment. These results indicate that proteins cross-linked to DNA by HN2 are bound to alkylated purines. Analysis of the DNA-bound proteins on NaDodSO4-polyacrylamide gels showed that primarily large nonhistone proteins are cross-linked to DNA in cells treated with HN2. Very little if any histone is cross-linked to the DNA. Comparison of DNA bound proteins from HN2-treated cells and HN2-treated nuclei showed that in general the same proteins are linked to DNA in both cases, but some qualitative and quantitative differences exist.
在用氮芥（HN2）处理细胞或分离的细胞核后，与DNA交联的蛋白质在CsCl梯度中进行纯化。蛋白质-DNA交联可以通过在稀酸中孵育来切割，并且可以通过碱预处理来稳定。这些结果表明，通过HN2与DNA交联的蛋白质与烷基化嘌呤结合。在NaDodSO4-聚丙烯酰胺凝胶上对与DNA结合的蛋白质进行分析表明，在用HN2处理的细胞中，主要是大型非组蛋白与DNA交联。几乎没有组蛋白与DNA交联。比较来自HN2处理细胞和HN2处理细胞核的与DNA结合的蛋白质表明，一般来说，两种情况下与DNA相连的是相同的蛋白质，但存在一些定性和定量的差异。
