Klug M J, Markovetz A J
J Bacteriol. 1968 Oct;96(4):1115-23. doi: 10.1128/jb.96.4.1115-1123.1968.
Candida lipolytica (Phaff) was grown in a mineral-salts medium amended with either 1-hexadecene or 1-heptadecene as substrate. Intermediates of the same chain length as the substrate were isolated and identified by various analytical procedures. The following intermediates of 16 and 17 carbon atoms were identified: omega-unsaturated acids, omega-unsaturated primary and secondary alcohols, 1,2-epoxides, 1,2-diols, and 2-hydroxy acids. Based on the chemical structure of these compounds, three oxidative mechanisms are proposed for the degradation of long-chain 1-alkenes by this yeast: (i) methyl-group oxidation, (ii) double-bond oxidation, and (iii) subterminal oxidation.
解脂假丝酵母（Phaff）在添加了1-十六碳烯或1-十七碳烯作为底物的矿物盐培养基中生长。分离出与底物链长相同的中间体，并通过各种分析方法进行鉴定。鉴定出了以下含16和17个碳原子的中间体：ω-不饱和酸、ω-不饱和伯醇和仲醇、1,2-环氧化物、1,2-二醇和2-羟基酸。基于这些化合物的化学结构，提出了该酵母降解长链1-烯烃的三种氧化机制：（i）甲基氧化，（ii）双键氧化，和（iii）亚末端氧化。
