Cline M J, Swett V C
J Exp Med. 1968 Dec 1;128(6):1309-25. doi: 10.1084/jem.128.6.1309.
Monocytes isolated from the peripheral blood of tuberculin-positive and tuberculin-negative donors were exposed to PPD, extensively washed, and incubated with autologous or homologous lymphocytes. Lymphocyte transformation was measured morphologically and by incorporation of (14)C-labeled thymidine. Monocytes from tuberculin-positive subjects induced transformation of autologous lymphocytes in 19 of 29 experiments. Studies to define the optimal conditions of exposure to monocytes to PPD and to autologous lymphocytes showed that viable, metabolically intact monocytes are required. A ratio of only 1 monocyte to 100 lymphocytes sufficed to induce transformation; neutrophils were inactive. In general, PPD-sensitized monocytes failed to induce transformation of homologous lymphocytes from either tuberculin-positive or tuberculin-negative subjects. Direct contact between monocytes and lymphocytes was required for consistent transformation, and islands of transforming lymphocytes were observed around a central core of monocytes.
从结核菌素阳性和结核菌素阴性供体的外周血中分离出单核细胞，将其暴露于结核菌素纯蛋白衍生物（PPD），充分洗涤后，与自体或同源淋巴细胞一起孵育。通过形态学观察以及（14）C标记胸腺嘧啶核苷的掺入来测定淋巴细胞转化情况。在29项实验中的19项里，来自结核菌素阳性受试者的单核细胞诱导了自体淋巴细胞的转化。确定单核细胞暴露于PPD和自体淋巴细胞的最佳条件的研究表明，需要有活力、代谢完整的单核细胞。单核细胞与淋巴细胞的比例仅为1:100就足以诱导转化；中性粒细胞无活性。一般来说，PPD致敏的单核细胞不能诱导来自结核菌素阳性或结核菌素阴性受试者的同源淋巴细胞转化。单核细胞与淋巴细胞之间需要直接接触才能持续发生转化，并且在单核细胞的中央核心周围观察到了转化淋巴细胞岛。
