Lapidus M, Grant N H, Alburn H E
J Lipid Res. 1968 May;9(3):371-3.
An enzymatic system has been developed for the production of prostaglandin E(2) (PGE(2)) from arachidonic acid by extracts of sheep seminal vesicular glands. The presence of glutathione insures high yields. A new procedure for the purification of PGE(2) was also developed, based on the dialysis of the biosynthesized product at pH 8 and extraction of the dialysate at pH 3 with chloroform. This procedure routinely gives yields of PGE(2) of 25-37% (from arachidonic acid) with a purity of 90-100%. Additional analytical proof of the identity of PGE(2) was provided by physicochemical characteristics of the crystalline thiosemicarbazide derivative, which can be readily prepared under mild conditions.
已经开发出一种酶系统，用于通过绵羊精囊腺提取物从花生四烯酸生产前列腺素E(2)（PGE(2)）。谷胱甘肽的存在确保了高产量。还开发了一种纯化PGE(2)的新方法，该方法基于在pH 8下对生物合成产物进行透析，并在pH 3下用氯仿萃取透析液。该方法通常能从花生四烯酸中获得25-37%的PGE(2)产量，纯度为90-100%。结晶硫代氨基脲衍生物的物理化学特性提供了PGE(2)身份的额外分析证据，该衍生物可在温和条件下轻松制备。
