依他尼酸对单个蟾蜍卵母细胞钠外流的作用。
The action of ethacrynic acid on sodium efflux from single toad oocytes.
作者信息
Bittar E E, Dick D A, Fry D J
出版信息
J Physiol. 1968 Jun;196(3):693-701. doi: 10.1113/jphysiol.1968.sp008530.
Abstract
- Ethacrynic acid (10(-3)M) at pH 7.4 caused approximately 83% inhibition of Na efflux from single dissected toad oocytes.2. Less inhibition was found at concentrations below 10(-3)M but higher concentrations did not cause greater inhibition.3. At an external pH of 7.0 the onset of inhibition was more rapid than at pH 7.4.4. Ouabain (10(-3)M) produced only 30% inhibition of the Na efflux, and the subsequent application of ethacrynic acid largely inhibited the remaining efflux.5. Application of ouabain, after ethacrynic acid had produced some 80% inhibition, caused only a further 9% drop in efflux.6. In uninhibited cells, Na efflux was found to be greater at pH 8.0, and less at pH 7.0 than at 7.4.
摘要
在pH 7.4条件下，依他尼酸（10⁻³M）使单个解剖蟾蜍卵母细胞的钠外流受到约83%的抑制。
在低于10⁻³M的浓度下，抑制作用较小，但更高浓度并不会导致更大的抑制。
在外部pH为7.0时，抑制作用的起始比pH 7.4时更快。
哇巴因（10⁻³M）仅使钠外流受到30%的抑制，随后应用依他尼酸可大大抑制剩余的外流。
在依他尼酸产生约80%的抑制作用后应用哇巴因，仅使外流进一步下降9%。
在未受抑制的细胞中，发现钠外流在pH 8.0时更大，在pH 7.0时比在pH 7.4时更小。
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