一种用于节肢动物蜕皮激素的放射免疫测定法，介绍了一种免疫原偶联的新方法。

A radioimmunoassay for arthropod moulting hormones, introducing a novel method of immunogen coupling.

作者信息

Spindler K D, Beckers C, Gröschel-Stewart U, Emmerich H

出版信息

Hoppe Seylers Z Physiol Chem. 1978 Oct;359(10):1269-72. doi: 10.1515/bchm2.1978.359.2.1269.

DOI:10.1515/bchm2.1978.359.2.1269

Abstract

Inokosteron-26-oic acid was coupled to thyroglobulin in aqueous pyridine by a water-soluble carbodiimide. After exhaustive dialysis and gel filtration on Sephadex G-25 in the presence of sodium dodecylsulfate, a coupling ratio of 164 haptens per molecule of thyroglobulin was determined. In all three animals injected with the conjugate, ecdysone-binding antibodies were detected. After one booster injection the antiserum could be diluted 1 : 5000 (1 : 4000, or 1 : 2000) in order to get a 50% binding of [3H]ecdysone. The dissociation constant was calculated as 5.8 X 10(-10) MOL/L. The antiserum has a greater affinity for ecdysone and 22-isoecdysone than for all other ecdysteroids and steroids tested.

摘要

通过水溶性碳二亚胺，将Inokosteron - 26 - 酸在吡啶水溶液中与甲状腺球蛋白偶联。在十二烷基硫酸钠存在下，经充分透析并在Sephadex G - 25上进行凝胶过滤后，测定每分子甲状腺球蛋白的偶联比为164个半抗原。在注射了该偶联物的所有三只动物中，均检测到蜕皮激素结合抗体。一次加强注射后，抗血清可稀释至1:5000（1:4000或1:2000），以实现[3H]蜕皮激素50%的结合。解离常数计算为5.8×10⁻¹⁰摩尔/升。该抗血清对蜕皮激素和22 - 异蜕皮激素的亲和力高于所有其他测试的蜕皮甾类和甾类。