Isenberg G, Small J V, Kreutzberg G W
J Neurocytol. 1978 Oct;7(5):649-61. doi: 10.1007/BF01260896.
In response to concanavalin A (Con A), neuroblastoma cells undergo marked morphological changes which involve the retraction of neurites and the induction of broad and extensive lamellar regions around the cell periphery. From the use of FITC-Con A it was shown that the membrane formed on the induced lamellar regions lacked receptors to Con A from the onset of lamella formation. These receptors were confined to the cell body; they initially showed a uniform distribution and were subsequently collected into patches and finally into aggregates or caps. When the aggregates occurred on the cell periphery their position coincided with areas free of lamellae. Investigations of the lamellar regions in Triton-extracted cell monolayers showed them to consist of a meshwork of actin filaments containing radiating thin filament bundles or microspikes. With increasing time in the presence of Con A there was a progressive increase in the number of radiating microspikes. Previous studies have shown the actin in these lamellar regions to be singly polarized with respect to the cell body. From the segregation of Con A receptors away from areas of actin polymerization in the lamellae it is concluded that actin is involved in some indirect way in surface receptor movement.
响应伴刀豆球蛋白A（Con A）时，神经母细胞瘤细胞会发生明显的形态变化，包括神经突回缩以及在细胞周边诱导形成宽阔且广泛的片状区域。通过使用异硫氰酸荧光素标记的伴刀豆球蛋白A（FITC-Con A）表明，在诱导形成的片状区域形成的膜从片状结构形成开始就缺乏对Con A的受体。这些受体局限于细胞体；它们最初呈现均匀分布，随后聚集成斑块，最终形成聚集体或帽状结构。当聚集体出现在细胞周边时，它们的位置与无片状结构的区域重合。对经曲拉通处理的细胞单层中的片状区域进行研究表明，它们由肌动蛋白丝网络组成，其中包含放射状的细丝束或微刺。在Con A存在的情况下，随着时间的增加，放射状微刺的数量逐渐增加。先前的研究表明，这些片状区域中的肌动蛋白相对于细胞体是单极化的。从Con A受体与片状结构中肌动蛋白聚合区域的分离可以得出结论，肌动蛋白以某种间接方式参与表面受体的移动。
