大豆脱辅基血红蛋白与原卟啉IX、血红素和染料的光氧化作用。

Photooxidation of soybean apoleghemoglobin with protoporphyrin IX, heme and dye.

作者信息

Perttilä U, Ellfolk N

出版信息

Eur J Biochem. 1978 Nov 15;91(2):335-8. doi: 10.1111/j.1432-1033.1978.tb12684.x.

DOI:10.1111/j.1432-1033.1978.tb12684.x

Abstract

Soybean apoleghemoglobin a was irradiated with visible light in the presence of different sensitizers to probe the heme environment of the protein. With protoporphyrin IX as sensitizer, specific photooxidation of histidine-92 and histidine-61 occurred. Irradiation of oxyleghemoglobin and cyanleghemoglobin resulted in photooxidation of histidine-92, while addition of methylene blue caused both histidine-92 and histidine-61 to be oxidized. Apoleghemoglobin, irradiated in the presence of rose bengal or methylene blue, lost tryptophan-128 in addition to the two histidines.

摘要

在不同敏化剂存在的情况下，用可见光照射大豆脱辅基血红蛋白a，以探究该蛋白质的血红素环境。以原卟啉IX作为敏化剂时，组氨酸-92和组氨酸-61发生特异性光氧化。对氧合血红蛋白和氰合血红蛋白进行照射会导致组氨酸-92发生光氧化，而添加亚甲蓝则会使组氨酸-92和组氨酸-61都被氧化。在孟加拉玫瑰红或亚甲蓝存在的情况下照射脱辅基血红蛋白，除了上述两个组氨酸外，色氨酸-128也会损失。