薄切片中肌动蛋白丝极性的可视化。膜相关丝均匀极性的证据。
The visualization of actin filament polarity in thin sections. Evidence for the uniform polarity of membrane-associated filaments.
作者信息
Begg D A, Rodewald R, Rebhun L I
出版信息
J Cell Biol. 1978 Dec;79(3):846-52. doi: 10.1083/jcb.79.3.846.
Abstract
We have developed an improved method for visualizing actin filament polarity in thin sections. Myosin subfragment-1 (S-1)-decorated actin filaments display a dramatically enhanced arrowhead configuration when fixed in a medium which contains 0.2 % tannic acid. With the exception of brush borders from intestinal epithelial cells, the arrowhead periodicity of decorated filaments in a variety of nonmuscle cells is similar to that in isolated myofibrils. The periodicity of decorated filaments in brush borders is significantly smaller. Actin filaments which attach to membranes display a clear, uniform polarity, with the S-1 arrowheads pointing away from the plasma membrane, while those which comprise the stress fibers of myoblasts and CHO cells have antiparallel polarities. These observations are consistent with a sliding filament mechanism of cell motility.
摘要
我们开发了一种改进的方法来观察薄切片中肌动蛋白丝的极性。当固定在含有0.2%单宁酸的介质中时，用肌球蛋白亚片段-1（S-1）标记的肌动蛋白丝会呈现出显著增强的箭头状结构。除了肠上皮细胞的刷状缘外，各种非肌肉细胞中标记丝的箭头状周期性与分离的肌原纤维中的相似。刷状缘中标记丝的周期性明显较小。附着于膜的肌动蛋白丝呈现出清晰、均匀的极性，S-1箭头远离质膜，而构成成肌细胞和CHO细胞应力纤维的肌动蛋白丝具有反平行极性。这些观察结果与细胞运动的滑动丝机制一致。
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