微量扩散法与离子交换法测定血浆铵的比较
Comparison between a microdiffusion and an ion exchange method for the determination of plasma ammonium.
作者信息
Acland J D, Strong R
出版信息
J Clin Pathol. 1968 Jan;21(1):12-3. doi: 10.1136/jcp.21.1.12.
The results of plasma-ammonium determinations by an ion-exchange method and by a microdiffusion method were compared on the same specimens with each other and with ammonium determinations carried out on the corresponding whole-blood specimens by the microdiffusion method. The plasma-ammonium levels of whole-blood specimens were higher than those of the corresponding plasma specimens, but the difference was not constant. Recoveries of added NH(4) from plasma by the microdiffusion method ranged from 65 to 144%. Recoveries of NH(4) from the same specimens by the ion-exchange method ranged from 93 to 96%. Of the two, the ion-exchange method was the only one of value.
采用离子交换法和微量扩散法对相同标本进行血浆铵测定，并将两种方法的测定结果相互比较，同时与用微量扩散法对相应全血标本进行的铵测定结果进行比较。全血标本的血浆铵水平高于相应血浆标本的血浆铵水平，但差异并不恒定。用微量扩散法从血浆中回收添加的NH₄的回收率在65%至144%之间。用离子交换法从相同标本中回收NH₄的回收率在93%至96%之间。两者之中，离子交换法是唯一有价值的方法。
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